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KMID : 0381019770100010026
Korean Journal of Nutrition
1977 Volume.10 No. 1 p.26 ~ p.33
Idenrification of Sugar from Korean Ginseng Saponins by Acid-hydrolysis.


Abstract
In order to identify the sugars of saponin originated from Korean ginseng, experimentations were made on the four kinds of ginseng. The results are summarized as follows.
1. The starch content of Ginseng-tail, White-ginseng 3 and 6 years old-whole ginseng were 10.4, 31. 5, 8. 2, 25.6% and total sugar of its were 371. 0, 61. 5, 64. 5, 62.5¢¥o and ;free sugar were 7. 6, 10. 5, 11. 3, and 10.75%, respectively.
2. Saponins were separated from Ginseng-tail, White-ginseng, 3 and 6 years old-whole ginseng by modified SHIBATA method. 1001ng of crude saponin was used for the Thin layer Chromatography (TLC)and thirteen to twelve spots of saponin were isolated by double development (Solvent: CHCL3: McOH: H2O=65 : 35 : 10) and by two dimensional development. (Solvent: nBuOH: HOAC: H2O=4 : 1 : 5) The pattern of spot was not significantly different according to Ginseng sample.
3. Glucose was identified from the acid-hydrolyzate of .Ginseng-tail saponin by paper chromatography and isolated the unknown chromatogram seems to be pentose.
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